Best Hand Soap for Effective Handwashing
and Killing Bacteria
Kiran David Singapogu
I.
RESEARCH PROBLEM
The spread of
COVID-19 has made us more conscious of our need to be clean. For most of us, this means prioritizing the
washing of our hands with soap and water regularly. Recently during the process of washing my
hands, it crossed my mind that there are many variables that could play a part
in the effectiveness of this process.
II.
RESEARCH QUESTION
I wonder what
handwashing products are most effective for killing bacteria?
III.
BACKGROUND RESEARCH
In 1864,
Hungarian doctor Ignaz Semmelweis, while working a hospital maternity ward,
discovered that when a handwashing regimen was implemented, death rates
dropped. Unfortunately, other doctors
disliked the practice of handwashing and boycotted it. During the Crimean War, Florence Nightingale
implemented procedures for sanitization including handwashing. This lead to improved health for wounded
soldiers. Gradually, the public has become more aware of the need for
handwashing. However, it wasn’t until
relatively recently, in the 1980’s, that there has been a widescale push for
general handwashing when the United States Center for Disease Control and
Prevention endorsed hand hygiene guidelines which have been increasingly
understood and implemented by the public.
Handwashing
products such as hand soap, hand sanitizer and antibacterial handwipes work by
killing the bacteria. Antibacterial hand
soap breaks the outer layer of the bacteria and kills it. It is then rinsed off with running
water. Hand sanitizer works by shaking
up the bacteria and viruses and bacteria on the surface of our skin in almost
the same way an earthquake demolishes a building. The viral and bacterial particles are still
present on the surface of our hands but are no longer living because they have
been broken apart.
The United States Center for Disease
Control and Prevention provides guidance and recommendations for general
handwashing. It is recommended that individuals wash their hands before and
after eating, or preparing food, after using the bathroom, and in a variety of
other situations. They provide the
following guidelines for effective handwashing:
1.
Wet your hands with clean, running water (warm or
cold), turn off the tap, and apply soap.
2.
Lather your hands by rubbing them together with the
soap. Lather the backs of your hands, between your fingers, and under your
nails.
3.
Scrub your hands for at least 20 seconds. Need a
timer? Hum the “Happy Birthday” song from beginning to end twice.
4.
Rinse your hands well under clean, running water.
5.
Dry your hands using a clean towel or air dry them.
IV.
HYPOTHESIS
Cleaning
hands with liquid antibacterial hand soap and water, will be the superior
method for handwashing compared to handwashing using bar soap, vinegar, hand
sanitizer or antibacterial wipes.
V.
PROCEDURE
1.
Take six pre-poured sterilized petri dishes. Using a Sharpie marker, label each petri dish
with a number one through six.
2.
Take petri dish labeled one and touch with
unwashed hands.
3.
Wash hands for twenty seconds (using a timer)
following CDC recommendations.
4.
With left hand, touch a light switch, doorknob,
writing utensil, tabletop and chair frame making maximum contact with each
object.
5.
Wash hands for twenty seconds (using a timer)
following above CDC recommendations using liquid antibacterial hand soap. Allow
hands to air dry.
6.
Touch petri dish number two using left hand
thumb, index finger and middle finger, making maximum possible contact.
7.
Repeat steps fourteen through sixteen for four
more trials and wash hands using: bar soap, vinegar, hand sanitizer and
antibacterial hand wipes and use petri dishes three through six.
8.
Cover petri dishes with lids and place in a clean
box. Store in a clean, dry location away from sunlight.
9.
Observe and photograph at noon daily.
VI.
MATERIALS
LIST
1.
Six petri
dishes
2.
Sharpie
3.
Timer
4.
Light switch
5.
Doorknob
6.
Writing utensil
7.
Tabletop
8.
Chair
9.
Liquid antibacterial hand soap
10. Bar soap
11. Vinegar
12. Hand sanitizer
13. Antibacterial wipes
VII.
RISK AND SAFETY
While
cultures are growing, petri dishes will be tightly sealed and stored in a
controlled environment so that no cultures are released into the environment.
It is
critical to dispose of used petri dishes properly. In order to destroy any cultures on petri
dishes a small amount of bleach solution will be applied prior to
disposal.
VIII.
DATA ANALYSIS
METHOD
I will take
pictures of each sample on the hour for seven days. At the end of seven days, I
will assess the growth of cultures using two methods. First, I will visually observe each petri
dish and count how many different kinds of cultures are visible and record the
number in a table. Second, I will
visually observe each petri dish and assess the percentage of the dish that is
covered in visible growth. I will record
the results in a table. After assessing
all handwashing agents by (1) visible number of cultures and (2) percentage of
coverage, I will calculate the final score of each handwashing agent.
IX.
BIBLIOGRAPHY
“Survey Reveals U.S. Handwashing Trends.” Professional Safety, vol.
65, no. 2, Feb 2020, p. 9.
Hunter, Beatrice Trum. “So, How Important Is Hand Washing?” Consumers’
Research Magazine, vol. 83, no. 4, Apr. 2000, p. 23.
Munson, Marty. “5 Myths About Hand Washing.” Prevention, vol.
70, no. 11, Nov. 2018, pp. 42-45.
Samonte, Pamela Rose V., and rhea U Vallente. Hand Hygiene
Compliance.” Salem Press Encyclopedia of Health, 2020.
Freeman, Matthew C., and Bethany A. Carusco. “Comment on ‘Global
Access to Handwashing: Implications for COVID-19 Control in Low-Income Contries.’”
Environmental Health Perspectives, vol. 128, no. 9, Sept. 2020.
“Improper Handwashing Spreads Bacteria.” USA Today Magazine, vol.
147, no 2879, Aug. 2018, pp. 4-5.
“Hand Sanitizer.” Britannica School, Encyclopedia Britannica, 22 Aug. 2019.
“The Global Handwashing Partnership.” Go to The Global Handwashing
Partnership., 2017,
globalhandwashing.org/about-handwashing/history-of-handwashing/.
“When and How to Wash Your Hands.” Centers for Disease Control and
Prevention, Centers for Disease Control and Prevention, 24 Nov. 2020,
www.cdc.gov/handwashing/when-how-handwashing.html.
X.
ANALYSIS OF DATA OR
RESULTS
According to my experiments, vinegar
performed best compared to other cleaning agents for handwashing. Hand
sanitizer and wipes tied as the second most effective cleaning agents. Liquid
hand soap was the third most effective cleaning agent and bar soap was the
least effective cleaning agent. As
expected, unwashed hands performed poorest in my experiment.
|
Trial 1
|
Trial 2
|
Trial 3
|
Average
|
Unwashed
hands
|
9
|
9
|
7
|
8.33
|
Liquid Hand soap
|
5
|
3
|
5
|
4.33
|
Bar
Soap
|
6
|
7
|
8
|
7
|
Vinegar
|
5
|
2
|
5
|
4
|
Hand
Sanitizer
|
4
|
5
|
4
|
4.33
|
Wipes
|
3
|
6
|
3
|
4
|

|
Number of cultures
|
Percentage of coverage
|
Final score
|
Unwashed hands
|
4
|
6
|
5
|
Liquid Hand soap
|
2
|
4
|
3
|
Bar Soap
|
3
|
5
|
4
|
Vinegar
|
1
|
1
|
1
|
Hand Sanitizer
|
2
|
2
|
2
|
Wipes
|
1
|
3
|
2
|

|
Trial 1
|
Trial 2
|
Trial 3
|
Average
|
Unwashed
hands
|
95%
|
90%
|
80%
|
88.3%
|
Liquid Hand soap
|
90%
|
15%
|
90%
|
65%
|
Bar
Soap
|
85%
|
50%
|
95%
|
77%
|
Vinegar
|
45%
|
15%
|
75%
|
45%
|
Hand
Sanitizer
|
50%
|
15%
|
95%
|
53%
|
Wipes
|
30%
|
55%
|
80%
|
55%
|

XI.
CONCLUSIONS
My hypothesis was that cleaning hands with
liquid antibacterial hand soap and water would be the superior method for
handwashing compared to handwashing using bar soap, vinegar, hand sanitizer or
antibacterial wipes. My results contradicted my hypothesis.
My
experiment went smoothly and I didn’t encounter any major problems. More trials would have helped to provide more
precise results. Additionally, it would
have been helpful to view my cultures under a microscope in order to more
precisely identify and quantify them.
I would like to
thank Mrs. Angie Mullinax and my parents for their assistance with this
research.